Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 979-985, 2013.
Article
de En
| WPRIM
| ID: wpr-247101
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts.</p><p><b>METHODS</b>Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.</p><p><b>RESULTS</b>The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.</p><p><b>CONCLUSION</b>The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.</p>
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Physiologie
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ARN messager
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Séquence nucléotidique
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Différenciation cellulaire
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Cellules cultivées
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Récepteur IGF de type 2
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Rat Sprague-Dawley
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Amorces ADN
/
Silice
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Biomedical and Environmental Sciences
Année:
2013
Type:
Article