Study on SNP polymorphism of mitochondrial DNA D-loop region from Nu ethnic population in Yunnan of China / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the mitochondrial single nucleotide polymorphism (SNP)of Chinese Nu ethnic population from Yunnan region of China and to provide basic database for ethnic origin investigation and forensic purpose.</p><p><b>METHODS</b>Genomic DNA from the whole blood of 87 unrelated individuals was extracted by standard chelex-100. The sequence polymorphism was analyzed by PCR-based assay and using ABI 3730 Analyzer to detect many number of relatively common point mutations.</p><p><b>RESULTS</b>Sixty-two SNP loci were observed among them with 492 point mutations and 59 haploids identified in mitochondrial DNA hypervariable region I (mtDNA HVSI). The gene diversity was estimated to be 0.9675,and the random match probability was calculated to be 0.0437.</p><p><b>CONCLUSION</b>The result suggests that mtDNA HVSISNP database of Nu ethnic population can be a useful tool for forensic identity and original research.</p>