Determination of mangiferin in rat plasma and aqueous humor by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 598-602, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-247424
ABSTRACT
A HPLC method was developed for the determination of mangiferin in rat plasma and aqueous humor. 4-Nitrophenol was used as internal standard. Analysis was performed on a Cosmosil ODS C18 analytical column (4.6 mm x 250 mm, 5 microm) with mobile phase consisting of methanol-water (40 60) with 2% glacial acetic acid at a flow rate of 1.0 mL x min(-1). The calibration curve of mangiferin in rat plasma and aqueous humor showed excellent linear behaviors over the investigated concentration of 0. 50-250.00 mg x L(-1) in plasma and 0.10-10.00 mg x L(-1) in aqueous humor, respectively, and the correlation coefficients were all above 0.995 4. The intra-day and inter-day precisions for all samples were measured to be below 12%. The limit of quantitation was 0.10 mg x L(-1) and low enough for the determination of mangiferin in all samples. The validated method has been successfully applied to preliminary pharmacokinetics study of mangiferin in rat plasma and aqueous humor.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Humeur aqueuse
/
Sang
/
Pharmacocinétique
/
Chimie
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Sensibilité et spécificité
/
Rat Wistar
/
Xanthones
/
Nitrophénols
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2011
Type:
Article
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