Preparation and properties of chitosan film as a drug sustained-release system / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 248-251, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-248259
ABSTRACT
<p><b>OBJECTIVE</b>To develop a best chitosan film for using as a drug sustained-release system through the evaluation of the sustained-release property, degradation property, and cytotoxicity to osteoblast.</p><p><b>METHODS</b>Orthogonal experiments were designed to determine the best combination of chitosan film preparations. Drug release rate was determined with Coomassie brilliant blue G250. In a separate study, chitosan films were placed into the test tubes with buffer solution and 10(7) U/L lysozyme. The degradation rate was calculated. Osteoblasts derived from fetal rat calvarial were cultured on chitosan films. Cell proliferation was tested by methyl thiazolyl tetrazolium (MTT) assay. The relative growth rate was calculated and the cytotoxicity was graded.</p><p><b>RESULTS</b>The best processing condition was 1% acetic acid, chitosan concentration of 2 mg/mL, 6% sodium tripolyphosphate (STPP) concentration, and cross-linking time of one hour. The resulting chitosan film released 33.13% of bovine serum albumin (BSA) within 8 d, 36.73% of BSA within four weeks and the cytotoxicity grade was 0 or 1.</p><p><b>CONCLUSION</b>This chitosan film possesses good sustained release property, and a good degradation rate.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polyphosphates
/
Sérumalbumine bovine
/
Préparations à action retardée
/
Chitosane
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2009
Type:
Article
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