Single nucleotide polymorphism loci of HIV-1 coreceptor CCR5 gene in Chinese Han people / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 72-74, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-248494
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the single nucleotide polymorphism(SNP) loci of HIV-1 coreceptor CCR5 gene in Chinese Han people.</p><p><b>METHODS</b>The coding region of CCR5 was amplified using 2 pairs of primers and the PCR products of all 42 healthy subjects were sequenced by 4 different primers. The results of sequencing were analyzed by DNAstar in search of SNP loci.</p><p><b>RESULTS</b>Six SNP loci were discovered in the coding region of CCR5, among them four SNPs, i.e. 184A-->G, 503G-->T, 688G-->A and 999G-->T, cause amino acids changes and two SNPs are nonsense mutations. One cytosine deletion at the 894nt results in frame shift mutation and prematured termination. 184A-->G, 503G-->T and 999G-->T were found in Chinese Han people for the first time. The allelic frequencies of mutant 184G, 503T and 999T alleles were 1.1%, 21.1% and 10.0% in healthy Hans, respectively. The population distribution of G503T markedly deviated from Hardy-Weinberg equilibrium.</p><p><b>CONCLUSION</b>The SNP loci in the coding region of CCR5 in Chinese Han people has its own characteristics, which is not consistent with those of Japanese and obviously different from those of Caucasian and African.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ADN
/
Analyse de mutations d'ADN
/
Séquence nucléotidique
/
Chimie
/
Chine
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
/
Délétion de séquence
/
Mutation ponctuelle
/
Récepteurs CCR5
/
Polymorphisme de nucléotide simple
Limites du sujet:
Adulte
/
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2003
Type:
Article
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