Cloning of human brain-derived neurotrophin-6 gene and its expression in procaryotic cell / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell.</p><p><b>METHODS</b>Total RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG).</p><p><b>RESULTS</b>The NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG.</p><p><b>CONCLUSION</b>The cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.</p>