Development and application of a two-step MacELISA for the early diagnosis of hemorrhagic fever with renal syndrome / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure.</p><p><b>METHODS</b>The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA.</p><p><b>RESULTS</b>This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%.</p><p><b>CONCLUSION</b>This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.</p>