Prokaryotic expression and purification of human papillomavirus type 11 L2E7 fusion protein vaccine and its immunnogenicity / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 156-158, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-248817
ABSTRACT
<p><b>OBJECTIVE</b>To construct the Escherichia coli (E. coli) prokaryotic expression system pET9aHPV11L2E7, purify the fusion protein L2E7 and study the immunnogenicity of the protein.</p><p><b>METHODS</b>The HPV11 L2, E7 coding region was amplified from condyloma acuminata tissue specimen by PCR. The recombinant plazmid pET9aHPV11L2E7 was established and sequenced. Fusion protein L2E7 (553 amino acids) was expressed in host strain BL21 (DE3plus) by IPTG inducing and identified by using SDS-PAGE and Western blotting. Then L2E7 protein purified with CM column was inoculated to Balb/c mice and its cell-mediated and humoral immunnogenicity was assessed by IFN-gamma enzyme-linked immunospot (ELISPOT) and enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The E. coli prokaryotic expression system pET9aHPV11L2E7 was established and the purified fusion protein L2E7 was obtained successfully. The mice in vivo experiment indicated that the purified protein L2E7 could induce HPV11E7 specific cell-mediated immune responses and high level HPV L2E7 antibody was detected in serum.</p><p><b>CONCLUSION</b>The purified fusion protein L2E7 could induce specific cell-mediated and humoral immune responses. It can be used as a candidate of genital wart immune therapeutic vaccine.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Sang
/
Protéines de fusion recombinantes
/
Expression des gènes
/
Protéines des oncogènes viraux
/
Infections à papillomavirus
/
Protéines de capside
/
Allergie et immunologie
/
Escherichia coli
/
Papillomavirus humain de type 11
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2007
Type:
Article
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