Effect of exogenous hydrogen sulfide on BACE-1 enzyme expression and β-amyloid peptide metabolism in high-glucose primary neuronal culture / 南方医科大学学报
Journal of Southern Medical University
;
(12): 504-510, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-249420
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of exogenous hydrogen sulfide (H2S) on β-site APP cleaving enzyme 1 (BACE-1) and β-amyloid peptide (Aβ) metabolism in primary culture of neurons under high-glucose condition.</p><p><b>METHODS</b>The cortical neurons in primary culture under normal and high glucose (60 mmol/L) conditions for 24 h were exposed to 25, 50 and 100 µmol/L NaHS. Aβ1-42 concentration in the cell culture was measured by ELISA, and BACE-1 mRNA and protein levels were detected by fluorescent quantitative real-time PCR and Western blotting, respectively.</p><p><b>RESULTS</b>Compared with the neurons cultured in normal glucose, the neurons exposed to high glucose showed significantly increased Aβ1-42 concentration and BACE-1 mRNA and protein expressions (P<0.05). Exposure to 25, 50 and 100 µmol/L NaHS significantly decreased Aβ1-42 concentration and BACE-1 mRNA and protein expressions in the high-glucose cell culture (P<0.05).</p><p><b>CONCLUSION</b>Neurons exposed to high glucose exhibit increased Aβ1-42 levels and BACE-1 mRNA and protein expressions, which can be concentration-dependently decreased by NaHS.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Fragments peptidiques
/
Pharmacologie
/
Cellules cultivées
/
Chimie
/
Peptides bêta-amyloïdes
/
Aspartic acid endopeptidases
/
Rat Sprague-Dawley
/
Milieux de culture
/
Amyloid precursor protein secretases
/
Glucose
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2014
Type:
Article
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