Application of Ac/Ds transposon system to genetate marker gene free transgenic plants in rice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 668-673, 2003.
Article
de Zh
| WPRIM
| ID: wpr-250010
Bibliothèque responsable:
WPRO
ABSTRACT
It is critical to generate marker gene free transgenic plants for retransformating or eliminating the potential harmfulness of marker gene and its product. In this study, Ac/Ds transposon system was developed for removal of hpt selection marker gene to obtain marker-free transgenic plants in rice ( Oryza sativa L.). Ds element containing the interesting gene bar was constructed next to the selection marker gene hpt to get Ds-T-DNA. Rice plants were transformed by Agrobacterium tumefaciens EHA105 containing Ac-T-DNA and Ds-T-DNA respectively. Rice plant containing single copy Ac-T-DNA was crossed with plant containing single copy Ds-T-DNA to obtain the F1 plant containing both Ac and Ds elements. F1 plant was self-crossed to produce F2 progeny in which T-DNA insert and transposed Ds element segregated independently. Two plants contained Ds element but no hpt marker gene in total 100 F2 plants. The result indicated that Ac/Ds transposon system could be used as a vector system for generating marker gene free transgenic plants in rice.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oryza
/
Transformation génétique
/
Éléments transposables d'ADN
/
Technique de Southern
/
Réaction de polymérisation en chaîne
/
Végétaux génétiquement modifiés
/
Agrobacterium tumefaciens
/
Croisements génétiques
/
Vecteurs génétiques
/
Génétique
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2003
Type:
Article