Inhibition of hepatitis B virus replication by RNA interference in vitro / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 522-525, 2004.
Article
de Zh
| WPRIM
| ID: wpr-250168
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To design pSilencer3.1-H1hygro plasmid expressing short interfering RNAs (siRNA) that targets HBV core gene region, and to evaluate inhibitory effect of this siRNA on HBV in vitro.</p><p><b>METHODS</b>HepG2 2.2.15 was used as target cells. The plasmid and liposome metafectene were cotransfected into the cultured cells, HBV DNA were analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR.</p><p><b>RESULTS</b>The plasmid expressing siRNA was successfully constructed. The two constructed siRNAs could effectively inhibit HBV replication, and their inhibitive effect on HBV was dose-dependent.</p><p><b>CONCLUSION</b>These results showed that siRNA could substantially inhibit HBV replication in the infected cells</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Physiologie
/
Virologie
/
Réplication virale
/
Cellules cancéreuses en culture
/
Virus de l'hépatite B
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Interférence par ARN
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Génétique
/
Tumeurs du foie
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Hepatology
Année:
2004
Type:
Article