Inhibition of hepatitis B virus replication by RNA interference in vitro / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To design pSilencer3.1-H1hygro plasmid expressing short interfering RNAs (siRNA) that targets HBV core gene region, and to evaluate inhibitory effect of this siRNA on HBV in vitro.</p><p><b>METHODS</b>HepG2 2.2.15 was used as target cells. The plasmid and liposome metafectene were cotransfected into the cultured cells, HBV DNA were analyzed by fluorogenic quantitative PCR (FQ-PCR), HBV C-mRNA was detected by semi-quantitative RT-PCR.</p><p><b>RESULTS</b>The plasmid expressing siRNA was successfully constructed. The two constructed siRNAs could effectively inhibit HBV replication, and their inhibitive effect on HBV was dose-dependent.</p><p><b>CONCLUSION</b>These results showed that siRNA could substantially inhibit HBV replication in the infected cells</p>