Construction and analysis of activity of an HIV-1/bovine immunodeficiency virus chimeric clone cDNA / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 143-145, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-250520
ABSTRACT
<p><b>OBJECTIVE</b>Chimeric human/bovine immunodeficiency virus (HBIV) cDNA was constructed by replacing HIV tat and LTR with bovine immunodeficiency virus (BIV) tat and LTR to study the activity of BIV tat and LTR in the chimerae.</p><p><b>METHODS</b>The target fragments of BIV tat, LTR and HIV gag, pol, env were respectively amplified by using PCR and sequentially inserted into pBluescript SK(+) vector. The chimeric clone was transfected into human MT4 cells. The transcript and gene expression of the HBIV chimeric virus were detected by using RT-PCR and a reverse transcriptase assay, respectively.</p><p><b>RESULTS</b>BIV tat mRNA and HIV gag mRNA were detected. The reverse transcriptase activity of the chimeric virus was analyzed in the fluctuation curve.</p><p><b>CONCLUSIONS</b>In chimeric HBIV cDNA transfected MT?4 cells, BIV tat and HIV gag were transcripted. The reverse transcriptase of the chimeric virus had biological activity. These data suggest that in MT4 cells, BIV LTR had promoter activity and BIV tat had the function of transactivation in the chimeric virus. The study of the chimeric virus with infectivity is in progress.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Transcription génétique
/
Réplication virale
/
Protéines de fusion recombinantes
/
ADN viral
/
Transfection
/
Gènes gag
/
Gènes pol
/
Gènes tat
/
Activation de la transcription
/
VIH-1 (Virus de l'Immunodéficience Humaine de type 1)
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2003
Type:
Article
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