Studies on the lignan chemical constituents from pine needles of Pinus massoniana Lamb / 药学学报

ABSTRACT

<p><b>AIM</b>To study the chemical constituents of the pine needles from Pinus massoniana Lamb..</p><p><b>METHODS</b>Various chromatographic techniques were used for the separation and purification. The physicochemical properties and spectral data were used to elucidate the structures.</p><p><b>RESULTS</b>Three compounds were isolated from the n-BuOH fraction of water-extracts. The structures were identified as (7S,8R)-4,9'-dihydroxyl-3, 3'-dimethyoxyl-7, 8-dihydrobenzofunan-1'-propanolneolignan-9-O-alpha- L-rhamnopyranoside (massonianoside D, 7), 4,4',9,9'-tetrahydroxyl-3,3'-dimethyoxyl-tetrahydrocyclolignan ((+)-isolariciresinol, 8) and 4,4',9'-trihydroxyl-3,3'-dimethyoxyl-tetrahydrocyclolignan-9-O-beta-D- xylopyranoside (isolariciresinol-9-O-xyloside, 9) on the basis of spectral data (ORD, UV, IR, MS, 1HNMR, 13CNMR, 1H-1HCOSY, HMQC and HMBC etc.).</p><p><b>CONCLUSION</b>Compound 7 is a new compound, while compounds 8 and 9 were isolated from this plant for the first time.</p>