Determination of tortoside A in Ilicis pubescentis by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of tortoside A in Ilex pubences.</p><p><b>METHOD</b>Kromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (1783), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT: Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%.</p><p><b>CONCLUSION</b>It was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.</p>