Correlation between expression level of functional genes and tanshinones' accumulation in Salvia miltiorrhiza from different areas / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3406-3409, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-251227
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between functional gene expression in Salvia miltiorrhiza from different producing areas and active principles, which might provide scientific basis for the gene regulation of tanshinones.</p><p><b>METHOD</b>The quantitative determination of cryptotanshinone and tanshinone II A was carried out by using HPLC method, expression level of 3 functional genes of SmAACT, SmCMK and SmIPPI were investigated by real-time PCR method.</p><p><b>RESULT</b>The content of active principles together with expression level of SmAACT and SmCMK were higher in S. miltiorrhiza from genuine producing areas including Henan and Shanxi, but lower in samples from Beijing which was non-genuine producing area.</p><p><b>CONCLUSION</b>Expression level of SmAACT and SmCMK had close relationships involving tanshinones' accumulation, but the SmIPPI gene had not.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Expression des gènes
/
Salvia miltiorrhiza
/
Abiétanes
/
Réaction de polymérisation en chaine en temps réel
/
Génétique
/
Métabolisme
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2011
Type:
Article
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