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Inhibition of α-glucosidase activity by water extracts of Xanthium sibiricum Patrin ex Widder and their effects on blood sugar in mice / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 632-637, 2013.
Article Dans Chinois | WPRIM | ID: wpr-251743
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Xanthium sibiricum Patrin ex Widder water extracts (CEW) on α-glucosidase activity (AG) and blood sugar in mice.</p><p><b>METHODS</b>The inhibition of AG by CEW was studied with enzyme-inhibitor screening external model with acarbose as control drug. The normal mice were administrated by gavage with 40.0g*kg(-1), or 10.0 g*kg(-1) of CEW, 0.375 g*kg(-1) of acarbose, and 0.3ml of normal saline, respectively in successive 5 days; then the animals were loaded with 2.0 g*kg(-1) of glucose, 4.0 g*kg(-1) of sucrose, and 2.0 g*kg(-1) of starch and blood sugar levels were measured within 15, 30, 60, and 120min. Diabetes was induced by injection of streptozotocin (STZ) in mice, then 40.0 g*kg(-1), or 10.0 g*kg(-1) of CEW was given to diabetic mice in successive 2 weeks and 4 weeks, then the blood sugar levels were measured.</p><p><b>RESULTS</b>In the enzyme inhibition test, when the concentration of CEW was between 0.3125 g*L(-1)-10.00 g*L(-1), the inhibition rate was 55.42%-92.73% when the concentration of acarbose was 1.5625 g*L(-1)-25.00 g*L(-1), the inhibition rate was 9.28%-64.87%. In the sugar tolerance test, the blood sugar value in starch-loaded mice decreased sharply (P<0.01), followed by sucrose-loaded group (P<0.05), and there was no change in glucose-loaded group (P>0.05). In diabetic mice CEW-40 and CEW-10 groups showed significant blood sugar lowering effect (P<0.01 or P<0.05).</p><p><b>CONCLUSION</b>CEW has stronger effect in inhibition of AG activity than acarbose. CEW can increase the sugar tolerance in normal mice and decrease the blood sugar level in diabetic mice..</p>
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / Glycémie / Extraits de plantes / Chimie / Xanthium / Diabète expérimental / Traitement médicamenteux / Antienzymes / Alpha-Glucosidase / Inhibiteurs des glycoside hydrolases Limites du sujet: Animaux langue: Chinois Texte intégral: Journal of Zhejiang University. Medical sciences Année: 2013 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / Glycémie / Extraits de plantes / Chimie / Xanthium / Diabète expérimental / Traitement médicamenteux / Antienzymes / Alpha-Glucosidase / Inhibiteurs des glycoside hydrolases Limites du sujet: Animaux langue: Chinois Texte intégral: Journal of Zhejiang University. Medical sciences Année: 2013 Type: Article