Research advances of the influence factors of high level expression of recombinant protein in Pichia pastoris / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1644-1649, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-251841
ABSTRACT
Pichia pastoris is one of the most important systems used in the field of molecular biology for the expression of recombinant proteins. The system has advantages of high expression, high stability, high secretion, easy high-density fermentation and low cost. Many factors affect the expression of recombinant protein, such as gene copy number, codon usage preference, type of promoter, molecular chaperones, glycosylation, signal peptide and fermentation process. In this review, research advances of the above aspects are summarized, which lay a foundation for improving the expression of recombinant proteins in P. pastoris.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pichia
/
Glycosylation
/
Protéines recombinantes
/
Signaux de triage des protéines
/
Régions promotrices (génétique)
/
Chaperons moléculaires
/
Dosage génique
/
Fermentation
/
Métabolisme
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2014
Type:
Article
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