Determination of lobetyolin in root of Codonopsis tangshen from various cultivation areas by high-performance liquid chromatography / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas.</p><p><b>METHOD</b>A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (2080) as the mobile phase and UV detection was at 268 nm.</p><p><b>RESULT</b>Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1).</p><p><b>CONCLUSION</b>The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.</p>