Effects of Bushen Shuji Granule on IL-6 in the culture fluid sample of fibroblast cells from the synovial liquid of the ankylosing spondylitis patients / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 257-260, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-252565
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of Bushen Shuji Granule (BSG) on inhibiting the interleukin 6 (IL-6) level in the synovial fluid sample of fibroblast cells from the ankylosing spondylitis (AS) patients.</p><p><b>METHODS</b>Using serum pharmacologic method, the IL-6 level in the culture fluid sample of fibroblast cells was observed by ELISA method with different concentrations of medicated serum containing BSG. The IL-6 level at the mRNA level was detected using reverse transcriptase-polymerase chain reaction (RT-PCR). The vehicle serum and sulfasalazine (SSZ) serum were taken as controls.</p><p><b>RESULTS</b>Results of ELISA showed the IL-6 level in the AS group was more than that in the vehicle serum group with obvious statistical difference. BSG could obviously inhibited the IL-6 level, showing statistical difference when compared with the vehicle serum group. Besides, obvious dose-dependent correlation existed between BSG and its inhibition on fibroblast proliferation. And the IL-6 level at the mRNA level in the AS group was higher than that in the vehicle serum group, showing statistical difference by semi-quantitative analysis.</p><p><b>CONCLUSION</b>BSG could play its clinical role of anti-inflammation and anti-fibrosis through inhibiting the IL-6 level in the culture fluid sample of fibroblast cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Pelvispondylite rhumatismale
/
Synovie
/
Membrane synoviale
/
ARN messager
/
Médicaments issus de plantes chinoises
/
Lignée cellulaire
/
Interleukine-6
/
Rat Sprague-Dawley
/
Sécrétions corporelles
Limites du sujet:
Animaux
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2012
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS