The assay of transcriptional activation effect of TR3 and deletion mutation in the yeast two hybrid system / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 504-507, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-252727
ABSTRACT
<p><b>AIM</b>To assay the transcriptional activation effect of TR3 and it's deletion mutation in yeast two hybrid system.</p><p><b>METHODS</b>The total length of TR3 and TR3/delta1-690 gene was amplified by PCR method and cloned into pGBKT7 vector. Bait vector of pGBKT7-TR3 and pGBKT7-TR3/delta1-690 was transformed into AH109 competence yeast. Then self activation of the recombination vector was tested by assay the activity of beta-galactosidae.</p><p><b>RESULTS</b>The pGBKT7-TR3 and pGBKT7-TR3/AM 690 vector was successfully constructed. The filter paper containing beta-galactosidae didn't changed to blue showed that the reporter gene wasn't activationed.</p><p><b>CONCLUSION</b>TR3 and TR3/delta1-690 hadn't the activity of transcriptional activation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Activation de la transcription
/
Délétion de séquence
/
Techniques de double hybride
/
Membre-1 du groupe A de la sous-famille-4 de récepteurs nucléaires
/
Génétique
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2008
Type:
Article
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