Protection mechanisms of ATP-sensitive K channels on hippocampal CA1 neurons during chronic severe hypoxia / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 257-261, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-253430
ABSTRACT
<p><b>AIM</b>To study the protection mechanisms of K(ATP) channels on hippocampal CA1 neurons during chronic severe hypoxia.</p><p><b>METHODS</b>p53 expression, DNA fraction, and cell apoptosis were examined in cultured hippocampal neurons in control group, hypoxia group, hypoxia group treated with K(ATP) channels antagonist and hypoxia group treated with K(ATP) channels agonist.</p><p><b>RESULTS</b>In the group of a 12 h long exposure to oxygen concentration of 0%, diazoxide (100 micromol/L), the K(ATP) channels agonist, reduced p53 expression and the hypoxia-induced apoptosis. In contrast, tolbutamide (100 micromol/L), the K(ATP) channels antagonist, significantly rose p53 expression and the hypoxia-induced apoptosis, which could be reversed by p53 inhibitor TSA.</p><p><b>CONCLUSION</b>K(ATP) channels protect hippocampal neurons against chronic severe hypoxia by suppressing p53 expression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Tolbutamide
/
Hypoxie cellulaire
/
Survie cellulaire
/
Adénosine triphosphate
/
Gènes p53
/
Rat Sprague-Dawley
/
Apoptose
/
Techniques de patch-clamp
/
Biologie cellulaire
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2007
Type:
Article
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