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Effect of protooncogene c-myb on progesterone-induced mouse germinal vesicle stage oocyte maturation in vitro / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 221-226, 2007.
Article Dans Chinois | WPRIM | ID: wpr-253440
ABSTRACT
<p><b>AIM</b>To investigate the effects of c-myb on progesterone-induced mouse germinal vesicle(GV) stage denuded oocyte (DO) maturation in vitro.</p><p><b>METHODS</b>We used mouse GV stage oocyte cultured with special concentration progesterone, or/and antisense c-myb ODN, or/and db-cAMP, or/and heparin for 24 h, and observed oocyte maturation and analysed the relationship among them.</p><p><b>RESULTS</b>We cultured DO in the medium 199 for 24 h, and found 10 micromol/L progesterone had more significant effect than 5 micromol/L progesterone (2 h GVBD% P < 0.05, 8 h PB 1% P < 0.05), but had not more significant effect than 20 micromol/L progesterone. We found that 16 micromol/L antisense c-myb ODN significantly inhibited progesterone (10 micromol/L)-induced mouse germinal vesicle stage oocyte maturation in vitro (2 h GVBD% P < 0.05, 8 h PBI% P < 0.01). 1 x 10(-4) micromol/L dbcAMP, 100 microg/ml heparin could single significantly inhibited progesterone-induced mouse GV stage oocyte maturation in vitro (2 h PBI% all P < 0.01, 8 h PBI% all P < 0.01), and could enhanced the inhibition of 16 micromol/L antisense c-myb ODN (2 h GVBD% all P < 0.01, 8h PBI% all P < 0.01).</p><p><b>CONCLUSION</b>Progesterone, protooncogene c-myb,cAMP and calcium all pay important role in regulating oocyte maturation and the mechanism of progesterone, cAMP and calcium in regulating oocyte maturation may be through the expression of protooncogene c-myb.</p>
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Ovocytes / Ovogenèse / Pharmacologie / Progestérone / Cellules cultivées / Gènes myb / Biologie cellulaire / Méiose / Lignées consanguines de souris Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Applied Physiology Année: 2007 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Ovocytes / Ovogenèse / Pharmacologie / Progestérone / Cellules cultivées / Gènes myb / Biologie cellulaire / Méiose / Lignées consanguines de souris Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Applied Physiology Année: 2007 Type: Article