Deposition of insulin powders for inhalation in vitro and pharmacodynamic evaluation of absorption promoters in rats / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1069-1074, 2005.
Article
Dans Anglais
| WPRIM
| ID: wpr-253529
ABSTRACT
<p><b>AIM</b>To prepare insulin powder for inhalation by spray-drying technology, determine the deposition of the insulin powder formulation in vitro and preliminarily investigate hypoglycemic response of the dry powder with/without absorption promoters.</p><p><b>METHODS</b>The depositions of the insulin powder for inhalation were determined by the China Pharmacopoeia 2000 version addenda XH and hypoglycemic effects were evaluated by testing serum glucose with glucose oxidase-peroxidase (GOD-PAP) method.</p><p><b>RESULTS</b>The depositions of the spray-dried insulin powder for inhalation were more than 40% under various humidity and their changes were not significant when air flow was no less than 18 L x min(-1). The coadministration of insulin with 8 mmol x L(-1)/dose sodium taurocholate [PA = 59.91%, Cnadir = (33 +/- 6) %] and 10 mmol x L(-1)/dose sodium deoxycholate [PA = 47.46% , Cnadir = (32 +/- 7)%] induced a significantly greater decline in blood glucose levels, while coadministration with 1% sodium caprylate, 1% sodium dodecyl sulfate, 250 microg/dose lecithin, 10 mmol x L(-1)/dose EDTA appeared to have no significant effect (P > 0.05).</p><p><b>CONCLUSION</b>Insulin powder for inhalation was relatively stable under various humidity conditions and different flow current. The use of 8 mmol x L(-1)/dose sodium taurocholate and 10 mmol x L(-1)/dose sodium deoxycholate could be able to potentially improve the bioavailability of insulin by pulmonary route.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Poudres
/
Acide taurocholique
/
Glycémie
/
Administration par inhalation
/
Biodisponibilité
/
Inspiration
/
Rat Sprague-Dawley
/
Acide désoxycholique
/
Synergie des médicaments
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2005
Type:
Article
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