The expression of Sonic Hedgehog during late bell stage by Western Blot and in situ hybridization / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression and biological effect of Shh during late bell stage by morphological and semi-quantitative analysis.</p><p><b>METHODS</b>Tooth germs were selected from new born Bal b/c mouse (P1, P2, P3, P5, P7). Semi-quality of Shh was measured by Western Blot and the expression place and strength of Shh were observed by in situ hybridization.</p><p><b>RESULTS</b>Shh was expressed in the ameloblast layer during late bell stage; the expression strength was high in secretive period and decreased with development; the active N-section was detectable before P3.</p><p><b>CONCLUSION</b>Shh expresses specially in the ameloblast layer in late bell stage, and expression quality is related to the function of ameloblasts.</p>