Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 287-289, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-254079
ABSTRACT
<p><b>OBJECTIVE</b>To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.</p><p><b>METHODS</b>TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.</p><p><b>RESULTS</b>TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.</p><p><b>CONCLUSION</b>HBX protein could be induced into mouse liver by TAT induced peptide.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Protéines de fusion recombinantes
/
Expression des gènes
/
Transactivateurs
/
Membrane cellulaire
/
Transport des protéines
/
Protéines à fluorescence verte
/
Escherichia coli
/
Produits du gène tat du virus de l'immunodéficience humaine
/
Protéines virales régulatrices ou accessoires
Limites du sujet:
Animaux
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2008
Type:
Article
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