Characterization of the cytopathic effect in human bronchial epithelial cell after Human Bocavirus Infection (HBoV) / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 107-109, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-254129
ABSTRACT
<p><b>OBJECTIVE</b>In this study, human bronchial epithelial cells were inoculated with positive sputum specimens of HBoV. After four days' infection, cytopathic effects (CPE) were observed by inverted microscopy. These viruses all cause typical cell damages such as rounded and shrivelled, fusion and fallout. These damages got quick following increased future degenerations. The other assay result of CPE within the infected cells were observed by inverted microscopy, have typical "owl's eye" plaque and above 90 percent hemadsorption within the infected cells by erythrocytes for hemadsorption technique. The typical fluorescence lump of nucleus within the infected cells was found by indirect immunofluorescence technique.</p><p><b>CONCLUSION</b>Isolation and identification of HBoV could be done in the human bronchial epithelial cell, and we found some characterizing CPE in the human bronchial epithelial cell after HBoV infection. The above studies pave a way for studying pathogenicity of human bocavirus.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Virologie
/
Bronches
/
Survie cellulaire
/
Cellules cultivées
/
Mort cellulaire
/
Technique d'immunofluorescence indirecte
/
Biologie cellulaire
/
Cellules épithéliales
/
Bocavirus
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2008
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS