The genetic stability of non-replicating recombinant adenoviruses on 293 cells during continuous passage / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 42-44, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-254146
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic stability during the serial passages of non-replicating recombinant adenoviruses based on the novel AdEasy system.</p><p><b>METHODS</b>Four non-replicating adenoviruses expressing rotavirus antigens, which were generated by the AdEasy system, were used as models and continuously propagated on 293 cells for 20 passages. Samples of the infected cells were collected at every 5 passages for the PCR analysis of the inserted rotavirus genes, replication-competent adenoviruses (RCAs) as well as the Western blot examination of the expression of the certain rotavirus genes.</p><p><b>RESULTS</b>The adenoviruses can be stably propagated on 293 cells. The existence and the stable expression of inserted rotavirus genes were able to be detected during the serial passage. The RCAs were not found within the first 10 passages, but the rvAdG2VP7(o) at passage 15 and the rvAdG2VP7(o) and rvAdG1VP7(o) at passage 20.</p><p><b>CONCLUSION</b>The non-replicating adenoviruses showed promising genetic stability during the continuous passage on 293 cells. The RCAs normally will not be detectable within 10 continuous passages. The results indicated the potential of such recombinant adenoviruses in the research and development of the gene therapies and adenoviral-vectored vaccines.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Réplication virale
/
ADN recombiné
/
Passages en série
/
Lignée cellulaire
/
Adenoviridae
/
Transgènes
/
Génétique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2008
Type:
Article
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