DNA extraction and sex determination for human teeth dated 3000 years ago unearthed in Xi'an / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 619-621, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-254549
ABSTRACT
<p><b>OBJECTIVE</b>To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi'an and determine the genders for the individuals.</p><p><b>METHODS</b>Thirty five ancient human teeth were studied. A 'Reverse-root-canal' technique and a Chelex-100 solution were used to extract the DNA. Specific primers for Amelogenin gene were designed for PCR amplification.</p><p><b>RESULTS</b>Genomic DNA was successfully extracted from 30 samples, for which 8 were determined to be males and 22 were females.</p><p><b>CONCLUSION</b>The 'Reverse-root-canal' technique may be used for extracting DNA from ancient human teeth. Genetics method can supplement physical anthropology for determination of sex for ancient samples.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Paléodontologie
/
Détermination du sexe
/
Dent
/
ADN
/
Chimie
/
Chine
/
Réaction de polymérisation en chaîne
/
Histoire ancienne
/
Amélogénine
/
Génétique
Limites du sujet:
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2013
Type:
Article
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