PI3K activity is associated with expression of neural specific genes in mouse fetal liver cells enhanced by butylated hydroxyanisole / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To study the mechanism of butylated hydroxyanisole-induced neural differentiation of fetal liver cells in vitro.</p><p><b>METHODS</b>14.5-day-old mouse fetal liver-derived cells were cultured, and were induced by 200 micromol/L butylated hydroxyanisole (BHA) combined with PI3K inhibitor LY294002 (20 micromol/L), and then were incubated in serum-free medium. Expression of genes in treated or untreated cells were assayed by Western blotting or RT-PCR.</p><p><b>RESULTS</b>There was low level of neurofilament-L (NF-L) and brain factor-1 (BF-1) but no neurofilament-H (NF-H) and tyrosine hydroxylase (TH) in fetal liver cells. BHA promoted significantly expression of neuron-specific NF-L, NF-H, BF-1, and TH in fetal liver cells. NF-L mRNA increased 5.8 fold, NF-H mRNA 8.0 fold, BF-1 mRNA 2.68 fold, and TH mRNA 30 fold, respectively (all P < 0.01 vs untreated cells). NF-L protein increased 11.29 fold, NF-H 5.5 fold, BF-1 2.53 fold, TH 4.76 fold. Moreover, expression of these BHA-induced genes were inhibited by PI3K inhibitor LY294002.</p><p><b>CONCLUSION</b>BHA induced neural differentiation of fetal liver cells through PI3K.</p>