Neuroprotective effects of paeonol in a cell model of Parkinson disease / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 30-36, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-255238
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of paeonol on neuron cell model of Parkinson disease (PD).</p><p><b>METHODS</b>The cell model of Parkinson disease was induced by treatment of 1-Methyl-4-phenylpyridinium (MPP+) in PC12 cells, the PD model cells were treated with 1 μmol/L, 3 μmol/L or 9 μmol/L paeonol for 24h, respectively. Cell viability and LDH leakage were detected by MTT and lactate dehydrogenase (LDH) assay; the apoptosis of PC12 cells was assessed by Hoechst 33258 staining and flow cytometry; reactive oxygen species (ROS) production was detected by DCFH-DA method; and the ratio of Bax/Bcl-2 and activation of caspase-3 were determined by Western blotting.</p><p><b>RESULTS</b>MPP+ treatment significantly reduced cell viability, increased LDH leakage, enhanced the proportion of apoptotic cells and ROS production. In addition, MPP+ treatment dramatically increased the Bax/Bcl-2 ratio, and the activation of caspase-3. Compared to PD model group, paeonol treatment significantly enhanced cell viability, decreased LDH leakage, inhibited the proportion of apoptotic cells and ROS production, reduced the Bax/Bcl-2 ratio and the activated caspase-3 protein.</p><p><b>CONCLUSION</b>Paeonol can prevent PC12 cells from apoptosis induced by MPP+, and the mechanism may be associated with the down-regulation of ROS production, Bax/Bcl-2 ratio and Caspase-3 activation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Maladie de Parkinson
/
Acétophénones
/
Pharmacologie
/
Régulation négative
/
1-Méthyl-4-phényl-pyridinium
/
Survie cellulaire
/
Cellules PC12
/
Espèces réactives de l'oxygène
/
Apoptose
/
Neuroprotecteurs
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2015
Type:
Article
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