Killing effect of sequential Herceptin and adriamycin treatment on breast cancer cell line in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 234-236, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-255345
ABSTRACT
<p><b>OBJECTIVE</b>To observe the killing effect of Herceptin and adriamycin sequentially applied on breast cancer cell line in vitro.</p><p><b>METHODS</b>BT-474 human breast cancer cells in exponential growth phase were treated with Herceptin alone, adriamycin alone and their sequential administration (Herceptin before adriamycin and vice versa), respectively. Under optical microscope, the morphological changes of the cells were observed before and after drug administration. The expression rate and mean fluorescence intensity (MFI) of HER-2/neu and cell death rate were detected by flow cytometry.</p><p><b>RESULTS</b>Microscopically, the cells treated with different protocols all exhibited such changes as darkening and increase of cellular debris with irregular cell morphology. Flow cytometry revealed no significant difference in the expression rate of HER-2/neu in each group before and after treatment, but the MFI of HER-2/neu and death rate of the treated cells were significant different from those of the control group (P<0.05). The cell death rate of Herceptin-pretreated cells was significantly higher than that of adriamycin-pretreated ones (P<0.05).</p><p><b>CONCLUSION</b>Herceptin pretreatment enhances the killing effect of adriamycin on breast cancer cell line BT-474, which provides experimental evidence for designing clinical sequential biochemotherapy of breast cancer.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Tumeurs du sein
/
Doxorubicine
/
Mort cellulaire
/
Récepteur ErbB-2
/
Lignée cellulaire tumorale
/
Synergie des médicaments
/
Anticorps monoclonaux humanisés
/
Cytométrie en flux
Type d'étude:
Guide de pratique
Limites du sujet:
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2006
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS