Expression of antisense thrombin receptor gene inhibits intimal hyperplasia of rat carotid artery after balloon injury / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 231-235, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-255401
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of restenosis after angioplasty and to clarify the effect of thrombin and its receptor on restenosis development.</p><p><b>METHODS</b>Balloon catheter-induced injury was adopted to induce intimal hyperplasia of the carotid arteries in rats. Antisense thrombin receptor (ATR) cDNA was transfected by perfusing recombinant LXSN ATR plasmid/nanoparticle complex into the segment of the injured carotid artery.</p><p><b>RESULTS</b>PCR result showed integration of the recombined gene. Dot blot showed the expression of antisense TR mediated by recombinant LXSN ATR plasmid/nanoparticle complex in the wall of common carotid arteries of the experimental group rats, which enabled to inhibit TR gene expression and intimal hyperplasia of the injured arteries.</p><p><b>CONCLUSIONS</b>Thrombin and its receptor play an important role in the formation of neointima after the injury, which provides a potential clue in developing a new approach for prevention and treatment of restenosis after angioplasty.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Thrombine
/
Artères carotides
/
Tunique intime
/
Récepteurs à la thrombine
/
Hyperplasie
/
Métabolisme
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Journal of Pathology
Année:
2002
Type:
Article
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