A study of E-cadherin and beta-catenin expression and their correlation with prognosis of nonsmall cell lung carcinoma / 中华病理学杂志
Chinese Journal of Pathology
; (12): 318-321, 2002.
Article
de Zh
| WPRIM
| ID: wpr-255424
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protein and mRNA expression of E-cadherin and beta-catenin in nonsmall cell lung carcinoma (NSCLC) and to find their correlation with histological type, differentiation, metastasis and prognosis.</p><p><b>METHODS</b>High sensitive S-P immunohistochemical method and in situ hibridization were used to detect the protein and mRNA expression of E-cadherin and beta-catenin.</p><p><b>RESULTS</b>Immunohistochemistry revealed that among the 101 cases, the positive rates of E-cadherin and beta-catenin were 68.3% and 81.2% respectively. The abnormal expression rates of these two proteins were 61.4% and 64.4% respectively. There was no significant relationship between E-cadherin and beta-catenin staining and histological type of the tumor (P > 0.05). However, there was a statistically significant difference between well and moderately differentiated cells and poorly differentiated cells (P < 0.05). In cases with lymphatic metastasis, the abnormal expression rates of E-cadherin and beta-catenin were significantly higher than those in nonmetastatic cases (P < 0.05). The mean survival time in cases with abnormal E-cadherin and beta-catenin expression were significantly shorter than that in cases with the expression grading (+ +) approximately (+ + +). In situ hybridization showed that in NSCLC, the positive rate of E-cadherin and beta-catenin mRNA was 38.9% and 47.2% respectively. Their concordant rates with (+ +) approximately (+ + +) protein expression were 78.6% and 82.4%, respectively.</p><p><b>CONCLUSIONS</b>The concordant rate of E-cadherin and beta-catenin mRNA and protein expression was relatively high. They can be used as markers of prognosis of NSCLC in clinical practice.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pronostic
/
ARN messager
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Immunohistochimie
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Cadhérines
/
Transactivateurs
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Chimie
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Taux de survie
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Mortalité
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Hybridation in situ
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Carcinome pulmonaire non à petites cellules
Type d'étude:
Prognostic_studies
Limites du sujet:
Adult
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Aged
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Pathology
Année:
2002
Type:
Article