Tree analysis pattern of mass spectral urine profiles in differential diagnosis of bladder transitional cell carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 274-277, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-255665
ABSTRACT
<p><b>OBJECTIVE</b>To develope a tree analysis pattern of mass spectral urine profiles to discriminate bladder transitional cell carcinoma (TCC) from non-cancer lesions using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology.</p><p><b>METHODS</b>Urine samples from 61 bladder transitional cell carcinoma (TCCs) patients, 53 healthy volunteers and 42 patients with other urogenital diseases were analyzed using IMAC-Cu-3 ProteinChip. Proteomic spectra were generated by SELDI-TOF- MS. A preliminary "training" set of spectra derived from analysis of urine from 46 TCC patients, 32 patients with benign urogenital diseases (BUD), and 40 age-matched unaffected healthy men were used to train and develop a decision tree classification algorithm which identified a fine-protein mass pattern that discriminated cancers from non-cancers effectively. A blinded test set including 38 cases was used to determine the sensitivity and specificity of the classification system.</p><p><b>RESULTS</b>The algorithm identified a cluster pattern that, in the training set, segregated cancer from non-cancer with a sensitivity of 84.8% and specificity of 91.7%. The discriminatory pattern was correctly identified. A sensitivity of 93.3% and a specificity of 87% for the blinded test were obtained when compared the TCC versus non-cancers.</p><p><b>CONCLUSION</b>SELDI-TOF-MS technology is a rapid, convenient and high-throughput analyzing method. The urine tree analysis proteomic pattern as a screening tool is effective for differential diagnosis of bladder cancer. More detailed studies are needed to further evaluate the clinical value of this pattern.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Hyperplasie de la prostate
/
Urine
/
Tumeurs de la vessie urinaire
/
Arbres de décision
/
Carcinome transitionnel
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Spectrométrie de masse MALDI
/
Cystite
/
Analyse par réseau de protéines
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Aged80
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2007
Type:
Article
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