Detection of micrometastases in sentinel lymph nodes of breast cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 116-118, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-255709
ABSTRACT
<p><b>OBJECTIVE</b>To investigate an optimal examination method to detect micrometastases in sentinel lymph nodes (SLNs) of breast cancer.</p><p><b>METHODS</b>Firstly, the SLNs of breast cancer were found by 99mTc-DX isotope method. Secondly, all the SLNs which were negative by routine HE examination were serially sectioned at a 100 microm interval and stained by both HE and immunohistochemistry for detecting micrometastases. All tumor tissue paraffin blocks were also sectioned and stained with HE and immunohistochemistry as control.</p><p><b>RESULTS</b>Totally, 121 SLNs and 44 tumors of 59 patients were examined. Micrometastasis was found to be positive in 17 SLNs (14.0%) of 14 patients (23.7%). When examined number of sections was increased from one to three, more positive micrometastatic SLNs were detected by HE staining only (3, 7, 10 for 1, 2, 3 sections, respectively). When HE staining was combined with immunohistochemical staining for AE1/3 or CK19 or muc1, much more positive micrometastatic SLNs were found (14, 12, 16 for 1, 2, 3 sections, respectively). The more sections were examined, the more micrometastases in SLNs were found. Furthermore, micrometastasis was also found to be positively correlated with the tumor size and the expression of c-erbB2, MMP-2, VEGF. The larger the tumor size was or the stronger expression of the above mentioned biomarkers, the more micrometastases in SLNs could be found.</p><p><b>CONCLUSION</b>Serially sections at a 100 microm interval and staining with both HE and immunohistochemical technique using muc1 antibody may be the best way to detect micrometastases in sentinel lymph nodes in breast cancer patients.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Tumeurs du sein
/
Imagerie diagnostique
/
Immunohistochimie
/
Scintigraphie
/
Composés organiques du technétium
/
Dextrane
/
Carcinome canalaire du sein
/
Récepteur ErbB-2
/
Matrix metalloproteinase 2
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2007
Type:
Article
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