Specific targeting cytotoxicity to resistant leukemia cells mediated by anti-Pgp/anti-CD3 diabody / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 342-344, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-255880
ABSTRACT
<p><b>OBJECTIVE</b>To study the specific targeting cytotoxicity to drug-resistant leukemia cells mediated by anti-Pgp/anti-CD3 diabody.</p><p><b>METHODS</b>The diabody was purified by affinity chromatography and identified by SDS-PAGE and FACS. The effect of the anti-Pgp/anti-CD3 diabody mediated lysis of Pgp-expressing tumor cells was assayed by human leukemia nude mice xenograft model in vivo.</p><p><b>RESULTS</b>The diabody was produced in E.coli in a soluble functional form and could bind both Jurkat cells (CD3(+)) and K562/A02 cells (Pgp(+)). The binding rates were 86.25% and 86.26%, respectively. It could inhibit tumor growth by 98.57% and prolonged the survival of mice bearing xenografted K562/A02 cells.</p><p><b>CONCLUSION</b>The diabody was proved to be a potent agent for mediating T lymphocyte cytotoxicity to lyse Pgp expressing tumor cells in vitro and in vivo.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Lymphocytes T
/
Antigènes CD3
/
Anticorps bispécifiques
/
Glycoprotéine P
/
Résistance aux médicaments antinéoplasiques
/
Cellules Jurkat
/
Tests d'activité antitumorale sur modèle de xénogreffe
/
Cytotoxicité immunologique
/
Allergie et immunologie
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2005
Type:
Article
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