Your browser doesn't support javascript.
loading
Spinal 5-HT3AR contributes to BmK I-induced inflammatory pain in rats / 生理学报
Acta Physiologica Sinica ; (6): 283-294, 2015.
Article Dans Anglais | WPRIM | ID: wpr-255947
ABSTRACT
Subcutaneous injection of BmK I could be adopted to well establish a novel pain model. Moreover, 5-hydroxytryptamine (serotonin, 5-HT) receptor is involved in regulating animal pain-related behaviors. However, the underlying mechanism of 5-HT3R on BmK I-induced pain remains unclear. Animal behavioral testing, RT-PCR and Western blotting were used to yield the following

results:

first, intraplantar (i.pl.) injection of BmK I (10 μg) induced elevated mRNA and protein levels of 5-HT3AR in bilateral L4-L5 spinal cord; Second, intrathecal (i.t.) injection of ondansetron (a specific antagonist of 5-HT3AR) reduced spontaneous pain responses, attenuated unilateral thermal and bilateral mechanical hypersensitivity elicited by BmK I; Microglia could be activated by BmK I (i.pl.) in both sides of L4-L5 spinal cord, and this effect was reversed by intrathecal pre-treatment with 5-HT3AR antagonist. Meanwhile, the 5-HT3AR in L4-L5 spinal cord was almost co-localized with NeuN (a marker of nerve cell), but not co-expressed with Iba-1 (a marker of microglia). Finally, the expression level of CX3CL1 and CX3CR1 was reduced by intrathecal pre-treatment with ondansetron. Our results indicate that both 5-HT3AR signaling pathway and microglia are activated in the process of induction and maintenance of BmK I-induced pain nociception. Meanwhile, our results suggest that the neuronal 5-HT3AR may communicate with microglia indirectly via CX3CL1 which is involved in regulating the BmK I-induced hyperalgesia and sensitization.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Venins de scorpion / Moelle spinale / Comportement animal / Injections rachidiennes / Rat Sprague-Dawley / Microglie / Récepteurs sérotoninergiques 5-HT3 / Chimiokine CX3CL1 / Hyperalgésie / Inflammation Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Acta Physiologica Sinica Année: 2015 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Venins de scorpion / Moelle spinale / Comportement animal / Injections rachidiennes / Rat Sprague-Dawley / Microglie / Récepteurs sérotoninergiques 5-HT3 / Chimiokine CX3CL1 / Hyperalgésie / Inflammation Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Acta Physiologica Sinica Année: 2015 Type: Article