The study on the stable inheritance and expression of foreign gene in transgenic mice / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 758-760, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-256124
ABSTRACT
Two transgenic mouse strains, in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32 +/- 5.41) microgram/mL in FIX-33 transgenic strain and (1.16 +/- 0.45) microgram/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P < 0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines recombinantes
/
Facteur IX
/
Souris transgéniques
/
Chimie
/
Hybridation fluorescente in situ
/
Lait
/
Génétique
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2002
Type:
Article
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