Expression of cre gene in Escherichia coli and bioassay its expression product / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 497-500, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-256177
ABSTRACT
The Cre recombinase from bacteriophage P1 can recognize specific DNA sequences, cleave DNA at specific target sites, and then ligate it to the cleaved DNA of a second site. In this study, cre gene was cloned into the pGEM-T Easy vector via PCR procedure. Then the cre gene was inserted into an expression vector pET-29a and expressed in E. coli BL21 (DE3). A 38 kD soluble protein was expressed and named CRE. CRE was purified by DEAE-52 chromatography. Bioassay of the partially purified product showed that CRE can cleave the plasmid pGLGFP which contains two loxP site with the same direction.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plasmides
/
Protéines virales
/
Protéines recombinantes
/
Régulation de l'expression des gènes codant pour des enzymes
/
Chromatographie sur DEAE-cellulose
/
Integrases
/
Protéines à fluorescence verte
/
Escherichia coli
/
Génétique
/
Protéines luminescentes
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2002
Type:
Article
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