Expression of polyprotein of infectious bursal disease virus in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
; (12): 472-476, 2002.
Article
de Zh
| WPRIM
| ID: wpr-256182
Bibliothèque responsable:
WPRO
ABSTRACT
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Bombyx
/
Protéines virales
/
Virologie
/
Test ELISA
/
Expression des gènes
/
Technique de Western
/
Virus de la bursite infectieuse
/
Polyprotéines
/
Électrophorèse sur gel de polyacrylamide
/
Vecteurs génétiques
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2002
Type:
Article