Your browser doesn't support javascript.
loading
Influence of inhibited α7 nicotinic acetylcholine receptor gene expression on the production of β-amyloid peptide in SH-SY5Y cells / 中华病理学杂志
Chinese Journal of Pathology ; (12): 837-841, 2012.
Article Dans Chinois | WPRIM | ID: wpr-256280
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of inhibited α7 neuronal nicotinic acetylcholine receptor (nAChR) by small interference RNA (siRNA) in SH-SY5Y cells and to explore the connection of these changes with the β-amyloid precursor protein (APP) metabolism and the pathogenesis of Alzheimer's disease (AD).</p><p><b>METHODS</b>The siRNA of α7 nAChR was transfected into SH-SY5Y cells, and the expression of α7 nAChR and two subtypes of β-secretases (BACE1 and BACE2) at mRNA and protein levels was studied by real-time PCR and Western blot, respectively. The variation of Aβ(1-42) content was detected by ELISA.</p><p><b>RESULTS</b>As compared with controls, the expression of α7 nAChR at mRNA and protein levels in the SH-SY5Y cells transfected with the α7 nAChR siRNA were decreased by 84% and 79% (P < 0.01), respectively. The expressions of BACE1 mRNA and protein levels was increased by 527% and 71% (P < 0.01), respectively, while the expression of BACE2 decreased by 58% and 75% (P < 0.01), respectively. The Aβ(1-42) content increased by 208% (P < 0.01).</p><p><b>CONCLUSIONS</b>An inhibited α7 nAChR mRNA induced by siRNA may markedly stimulate the production of Aβ through the mechanism of increased expression of BACE1 and inhibited expression of BACE2, which may be related to the pathogenesis of AD.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Fragments peptidiques / ARN messager / Transfection / Peptides bêta-amyloïdes / Aspartic acid endopeptidases / Récepteurs nicotiniques / Petit ARN interférent / Interférence par ARN / Lignée cellulaire tumorale Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Pathology Année: 2012 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Fragments peptidiques / ARN messager / Transfection / Peptides bêta-amyloïdes / Aspartic acid endopeptidases / Récepteurs nicotiniques / Petit ARN interférent / Interférence par ARN / Lignée cellulaire tumorale Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Pathology Année: 2012 Type: Article