Effects of ezrin silencing on pancreatic cancer cell line Panc-1 / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 833-836, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-256281
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.</p><p><b>METHODS</b>Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid. The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis, respectively. Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy. Colony formation assay was used to determine the cell anchor-independent growth ability in vitro. Trans-filter migration and invasion assays were performed with 8 µm pore inserts in a 24-well BioCoat chamber with/without Matrigel.</p><p><b>RESULTS</b>Ezrin silencing decreased cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion, but had no effects on cell proliferation in vitro and cell cycle, in pancreatic cancer cell line Panc-1.</p><p><b>CONCLUSION</b>Ezrin expression affects the cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion in pancreatic cancer cell line Panc-1.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tumeurs du pancréas
/
Anatomopathologie
/
Plasmides
/
Transfection
/
Mouvement cellulaire
/
Prolongements cytoplasmiques
/
Protéines du cytosquelette
/
Petit ARN interférent
/
Interférence par ARN
/
Lignée cellulaire tumorale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Pathology
Année:
2012
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS