Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 440-445, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-257614
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice.</p><p><b>METHODS</b>A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each) the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery.</p><p><b>RESULT</b>The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05).</p><p><b>CONCLUSION</b>The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plaies et blessures
/
Test ELISA
/
Lésion d'ischémie-reperfusion
/
Chimiokines
/
Protéines et peptides de signalisation intercellulaire
/
Intestins
/
Ischémie
/
Souris de lignée C57BL
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2015
Type:
Article
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