The effects of sera from patients with paroxysmal nocturnal hematoglobinuria on the growth of CD34(+) cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 47-51, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-258071
ABSTRACT
To investigate the effects of sera on the growth of single CD34(+) cells from patients with paroxysmal nocturnal hematoglobinuria (PNH), sera from both PNH patients and normal individuals were added separately to the single cell culture system and semi-solid colony formation system. The growth of the normal CD34(+) and PNH CD34(+) CD59(+) and CD34(+) CD59(-) cells was evaluated. No growth difference was found for growth of the normal CD34(+) and PNH CD34(+) CD59(+) cells when PNH or normal sera were added to the culture media in either single cell culture or colony formation culture. While no difference was detected for single PNH CD34(+) CD59(-) cells growth when PNH or normal sera were added, more colonies were observed in semi-solid culture with PNH sera. A conclusion was reached that compared with those from normal controls, the sera from PNH patients had no significant influence on single hematopoietic stem/progenitor cells derived from normal subjects and from PNH patients, but the PNH sera might promote the colony formation of the CD34(+) CD59(+) cells in semi-solid culture
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Cellules souches hématopoïétiques
/
Protéines du sang
/
Division cellulaire
/
Cellules cultivées
/
Antigènes CD59
/
Antigènes CD34
/
Allergie et immunologie
/
Hémoglobinurie paroxystique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2002
Type:
Article
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