Your browser doesn't support javascript.
loading
Study of sequence variations of Epstein-Barr virus LMP1 gene in nasopharyngeal carcinoma / 中华病理学杂志
Chinese Journal of Pathology ; (12): 791-795, 2005.
Article Dans Chinois | WPRIM | ID: wpr-258258
ABSTRACT
<p><b>OBJECTIVE</b>To detect the sequence variations frequently found within the N- and C-terminal regions of Epstein-Barr virus (EBV) LMP1 gene in nasopharyngeal carcinoma (NPC) and to study the underlying mechanisms.</p><p><b>METHODS</b>Fresh tumor tissues were sampled from 63 patients with untreated NPC encountered in Affiliated Tumor Hospital of Sun Yat-sen University, Guangzhou. The N-terminal region of EBV LMP1 gene was amplified with nested polymerase chain reaction (PCR), followed by XhoI enzyme digestion. Nested PCR was also employed to detect the 30 base pairs deletion within the C-terminal region. Four-colored fluorescence terminator sequencing method was applied for bi-directional solid-phase sequencing of the 8 representative PCR products in 4 cases of NPC. The DNA sequence within the N- and C-terminal regions of LMP1 gene was then analyzed.</p><p><b>RESULTS</b>There were 4 patterns of sequence variations, namely, wt-XhoI/wt-LMP1 (4 cases, 6.3%), wt-XhoI and XhoI-loss/del-LMP1 (4 cases, 6.3%), wt-XhoI/del-LMP1 (5 cases, 7.9%) and XhoI-loss/del-LMP1 (50 cases, 79.5%), detected in the 63 studied cases. Sequence analysis showed that the EBV LMP1 gene had underwent non-synonymous and synonymous substitutions, as compared with the prototype of B95-8 cells. The ratio of non-synonymous to synonymous substitutions was 2.25.</p><p><b>CONCLUSIONS</b>XhoI-loss/del-LMP1 is the predominant sequence variation pattern of EBV LMP1 gene in NPC from Guangzhou. The XhoI-loss variation seems to develop on top of del-LMP1. When compared with the EBV LMP1 gene in peripheral blood B-lymphocytes of virus carriers and in preinvasive epithelial lesions (reported previously), it is likely that the sequence variation patterns of LMP1 gene may represent 4 different phases of intrahost evolution of EBV during nasopharyngeal carcinogenesis.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Variation génétique / Virologie / ADN viral / Données de séquences moléculaires / Séquence nucléotidique / Type II site-specific deoxyribonuclease / Protéines de la matrice virale / Tumeurs du rhinopharynx / Analyse de séquence d&apos;ADN / Mutation ponctuelle Limites du sujet: Adulte / Adulte très âgé / Femelle / Humains / Mâle langue: Chinois Texte intégral: Chinese Journal of Pathology Année: 2005 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Variation génétique / Virologie / ADN viral / Données de séquences moléculaires / Séquence nucléotidique / Type II site-specific deoxyribonuclease / Protéines de la matrice virale / Tumeurs du rhinopharynx / Analyse de séquence d&apos;ADN / Mutation ponctuelle Limites du sujet: Adulte / Adulte très âgé / Femelle / Humains / Mâle langue: Chinois Texte intégral: Chinese Journal of Pathology Année: 2005 Type: Article