Morphological study on the role of ICC-like cells in detrusor neuro-modulation of rat urinary bladder / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1542-1545, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-258328
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of ICC-like cells in bladder neuromodulation in rat urinary bladder.</p><p><b>METHODS</b>14 SD rats and 1 guinea pig were sacrificed in this study. The ultra structural relationships among interstitial cells, nerves and detrusor smooth muscle cells (DSMCs) of urinary bladder were investigated by transmission electron microscopy (TEM). c-kit immunofluorescence was used to identify ICC-like cells in SD rat urinary bladder and the structural relationship between ICC-like cells and nerve terminals was studied by immunofluorescence (double-label).</p><p><b>RESULTS</b>Gap junction between ICC-like cells and DSMCs was confirmed by TEM. ICC-like cells were very close apposition with nerve terminals under TEM. ICC-like cells were identified to exist in sub-urothelium layer, along the longitude of smooth muscle bundles and among detrusor smooth muscle in SD rat urinary bladder by c-kit immunofluorescence. Double-labeled tissue with c-kit and PGP9.5 antibodies also showed that ICC-like cells were very close apposition with nerve terminals in SD rat bladder.</p><p><b>CONCLUSIONS</b>Morphological study indicated that ICC-like cells in rat urinary bladder may play an important role in detrusor neuromodulation. Further study on function will be helpful for elucidating the mechanism of bladder neuromodulation clearly.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Vessie urinaire
/
Cellules cultivées
/
Rat Sprague-Dawley
/
Jonctions communicantes
/
Biologie cellulaire
/
Myocytes du muscle lisse
/
Cochons d'Inde
/
Muscles lisses
/
Terminaisons nerveuses
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Surgery
Année:
2008
Type:
Article
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