Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 374-378, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-258810
ABSTRACT
Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. The average recovery range was 88.5%-105.4% for spiked samples (10, 50, and 100 μg/kg), and the coefficient of variation was in the range of 7.5%-14.5%. The limit of detection of CLEIA was 9.4 μg/kg, and this method was compared with the liquid chromatography-tandem mass spectrometry method using naturally contaminated samples, producing a correlation coefficient of >0.95. We demonstrate a reliable CLIEA for the rapid screening of neomycin in milk.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Résidus de médicaments
/
Néomycine
/
Contamination des aliments
/
Chimie
/
Techniques immunoenzymatiques
/
Lait
/
Limite de détection
/
Mesures de luminescence
/
Métabolisme
/
Antibactériens
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2016
Type:
Article
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