Induction and culture of hairy boots in Phytolacca esculenta and its saponin production / 生物工程学报

ABSTRACT

Hairy roots appeared in vitro 20 days after inoculation of Phytolacca esculenta leaf explants with the strain of Agrobacterium rhizogenes R1601. The frequency of leaf explants transformed by R1601 was up to 70%. Hairy roots can be incited directly from the veins of explants or via callus. Hairy roots induced by R1601 grew rapidly on medium MS without hormone and were 85.6% higher in respiration rate than control roots. Transformation was confirmed by opine detection and the amplification of rol B and rol C genes from the hairy roots of P. esculenta. The total saponin content in hairy roots of P. esculenta was about 1.54 times as much as in natural roots whereas polysaccharides content was about 70% times as much as in the natural roots.