An in vitro hepatic steatosis cell model for study of non-alcoholic fatty liver disease / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To establish an in vitro cell model to investigate hepatic steatosis of non-alcoholic fatty liver disease.</p><p><b>METHODS</b>HepG2 cells cultured in MEM containing 10 % fetal bovine serum were divided into control group and model group. At 7 0%-80 % confluency, HepG2 cells in model group were exposed to a long-chain mixture of free fatty acids (oleate and palmitate) for 24 h, cells in control group were subject to fresh medium. Lipid droplets were observed with oil red O stain and electron microscope, triglyceride and malonaldehyde were detected by respective assay kits.</p><p><b>RESULT</b>A large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However,malonaldehyde did not increase significantly compared with control group.</p><p><b>CONCLUSION</b>A large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However, malonaldehyde did not increase significantly compared with control group.</p>