Effect of genistein on rat femoral bone metabolic activity in vitro / 药学学报
Acta Pharmaceutica Sinica
;
(12): 960-964, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-259523
ABSTRACT
This study is to investigate effects of genistein on rat femoral bone metabolic in vitro. Rat femoral tissues was isolated and randomly divided into two groups including control group and genistein (1 x 10(-5) mol x(-1)) group. Determinations of alkaline phosphatase (ALP) activity, calcium content and osteoprotegerin (OPG), type I-collagen (Collagen-I), RANKL, Runx-2 and bone morphogenetic protein (BMP-2) mRNA expression were done by real-time PCR. The results showed that 1 x 10(-5) mol x L(-1) genistein could increase the activity of ALP and contents of Ca, regulate bone metabolism activity of OPG, RANKL, BMP-2, Collagen-I and Runx-2 mRNA expression level. Genistein can significantly modulate bone metabolism related gene expression level of rat femoral tissue in vitro, and can increase calcium content and the activity of ALP.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
ARN messager
/
Répartition aléatoire
/
Régulation de l'expression des gènes
/
Calcium
/
Rat Sprague-Dawley
/
Génistéine
/
Collagène de type I
/
Phyto-oestrogènes
/
Phosphatase alcaline
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2013
Type:
Article
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